- generation of mouse mutants by homologous recombination in embryonic stem cells (gene targeting, inducible knock-out)
- variety of molecular biology methods including generation and screening of libraries, cloning of genes, in situ hybridization techniques, expression of recombinant proteins, blotting techniques
- in vitro cell culture assays with primary cells and genetically modified cell lines
- analysis of mutant mice by morphological, histological, and cytological techniques applied to paraffin, semi-thin, and ultrathin tissue-sections
- comparative analysis of protein and mRNA expression in mutant mice by immunocytochemical and in situ hybridization techniques
- behavioural analysis of mouse mutants
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